Compared with the traditional cyanine dyes (Cy5), both the maximum absorption and emission wavelengths of new Cy5 dyes with aldehyde group substituted at central position of the conjugated chain slightly blue shift, and the Stokes Shift is enlarged evidently to more than 28 nm, and largest up to 48 nm in water. The fluorescence quantum yield is rather low without obvious change in different solvents. It is found that the fluorescence of dye is sensitive to viscosity of the solvents, and the emission intensity of the dye can be enhanced largely by ascending of environmental viscosity with well linear relationship between lg If and lg (viscosity) (F?rster-Hoffmann equation). It is living cell membrane permeable for the dye 4 to map cellular microenvironmental viscosity, which demonstrates the potential application for dye 4 to investigate the intracellular microviscosity change.
杨志刚, 和艳霞, 戴彬, 窦柏蕊, 王静云, 彭孝军. 中位醛基取代Cy5的光谱性能及作为粘度荧光探针的研究[J]. 化学学报, 2011, 69(04): 445-451.
YANG Zhi-Gang, HE Yan-Xia, DAI Bin, DOU Bai-Rui, WANG Jing-Yun, PENG Xiao-Jun. The Spectral Properties of Cy5 with Aldehyde Group Substituted at Meso-position of the Polymethine Chain Utilized as Fluorescent Viscosity Sensor. Acta Chimica Sinica, 2011, 69(04): 445-451.