Abstract：The mechanism of color changes of Commassie Brilliant Blue G-250 (CBBG) has been investigated by spectrometry. The influence of different experimental conditions on the absorption spectra of CBBG was inspected. It is found that CBBG forms aggregate by hydrophobic interaction in high phosphoric acid concentration. Due to the difference in the aggregative degree, CBBG shows color changes from blue to green to brown in the system.
曹稳根,焦庆才,刘茜,陈雷. 考马斯亮蓝显色剂变色反应机理的研究[J]. 化学学报, 2002, 60(9): 1656-1661.
Cao Wengen;Jiao Qingcai;Liu Qian;Chen Lei. Study on the Mechanism of Color Changes of Commassie Brilliant Blue G-250. Acta Chimica Sinica, 2002, 60(9): 1656-1661.